AA_substitution enzyme_activity Qax-Ala SD(Qax-Ala) Qax-Fru-1,6-BP SD(Qax-Fru-1,6-BP) #Experiment_1 Wild Type 1 0.079 0.001 * * R55A 1 0.2 0.01 * * R55E 1 1 ND * * R55F 1 1 ND * * R55H 1 0.59 0.04 * * R55I 1 1 ND * * R55K 1 1 ND * * R55L 1 1 ND * * R55M 1 ND ND * * R55P 1 0.4 0.01 * * R55S 1 0.5 0.05 * * R55V 1 1 ND * * R55Y 0 ND ND * * S56A 1 0.13 0.01 * * S56C 0 ND ND * * S56G 1 0.3 0.1 * * S56I 1 0.46 0.04 * * S56K 1 0.39 0.02 * * S56L 1 0.24 0.02 * * S56R 1 0.27 0.02 * * S56T 1 0.014 0.001 * * S56W 1 0.09 0.01 * * N82A 1 0.52 0.02 * * N82C 1 0.12 0.01 * * N82D 1 ND ND * * N82G 1 0.3 0.1 * * N82H 0 ND ND * * N82K 0 ND ND * * N82L 1 1 ND * * N82R 1 1 ND * * N82S 1 0.18 0.01 * * N82T 1 0.025 0.02 * * N82V 1 0.12 0.01 * * N82W 0 ND ND * * N82Y 0 ND ND * * R118A 1 0.16 0.01 * * R118D 1 ND ND * * R118E 1 0.27 0.01 * * R118F 1 0.11 0.01 * * R118G 1 0.09 0.01 * * R118H 1 0.15 0.01 * * R118I 1 0.12 0.01 * * R118K 1 0.26 0.01 * * R118L 1 0.19 0.01 * * R118M 1 0.12 0.01 * * R118N 1 0.13 0.01 * * R118P 1 0.12 0.01 * * R118Q 1 0.17 0.01 * * R118S 1 0.15 0.01 * * R118T 1 0.16 0.01 * * R118V 1 0.18 0.01 * * H476A 1 ND ND * * H476D 1 1 ND * * H476F 1 1 ND * * H476I 1 ND ND * * H476K 1 1 ND * * H476L 1 1 ND * * H476N 1 ND ND * * H476Q 1 0.043 0.004 * * H476S 1 ND ND * * H476T 1 ND ND * * H476V 1 ND ND * * V481A 1 0.075 0.003 * * V481C 1 0.047 0.002 * * V481E 0 ND ND * * V481F 1 ND ND * * V481G 1 0.063 0.007 * * V481H 0 ND ND * * V481I 0 ND ND * * V481K 1 1 ND * * V481L 1 0.1 0.01 * * V481M 1 0.083 0.005 * * V481P 0 ND ND * * V481R 0 ND ND * * V481S 1 0.049 0.002 * * V481Y 0 ND ND * * F482A 1 1 ND * * F482E 1 1 ND * * F482H 1 0.27 0.02 * * F482I 1 1 ND * * F482K 1 1 ND * * F482L 1 1 ND * * F482M 1 1 ND * * F482Q 1 1 ND * * F482R 1 0.3 0.02 * * F482S 1 1 ND * * F482V 1 1 ND * * F482W 1 0.025 0.001 * * F482Y 1 0.097 0.003 * * P483A 0 ND ND * * P483C 0 ND ND * * P483D 0 ND ND * * P483E 0 ND ND * * P483G 1 ND ND * * P483I 0 ND ND * * P483K 0 ND ND * * P483L 0 ND ND * * P483T 0 ND ND * * P483V 0 ND ND * * P483W 0 ND ND * * F514A 1 0.48 0.03 * * F514D 1 1 ND * * F514E 0 ND ND * * F514G 1 ND ND * * F514I 1 0.41 0.01 * * F514L 1 0.21 0.01 * * F514M 1 0.38 0.01 * * F514N 1 0.56 0.03 * * F514P 1 0.47 0.03 * * F514R 1 0.37 0.06 * * F514S 1 0.47 0.05 * * F514T 1 0.42 0.01 * * F514W 1 0.34 0.02 * * F514Y 1 0.52 0.04 * * #Experiment_2 Wild Type 1 0.0822 0.0006 14.2 0.2 E2A 1 0.083 0.004 10.5 0.7 P4A 1 0.061 0.003 15 1 Y7A 1 0.066 0.004 13 1 L8A 1 0.095 0.003 33 5 R9A 1 0.054 0.004 23 3 R10A 1 0.062 0.002 21 2 S12A 1 0.061 0.006 7.7 0.8 V13A 1 0.099 0.004 25 2 Q15A 1 0.093 0.005 8.8 0.9 L16A 1 0.195 0.007 21 2 T17A 1 0.101 0.005 9.7 0.9 Q18A 1 0.035 0.002 5.5 0.4 E19A 1 0.112 0.008 21 4 L20A 1 0.274 0.004 36 1 T22A 1 0.073 0.003 8.3 0.3 F24A 1 0.322 0.008 31 1 F25A 1 0.347 0.005 21.7 0.6 Q26A 1 0.273 0.006 11.5 0.4 Q27A 1 0.087 0.002 19.8 0.7 Q28A 1 0.104 0.002 3.8 0.2 Q29A 1 0.112 0.002 47 1 L30A 1 0.132 0.003 28 2 P31A 1 0.064 0.002 3.6 0.2 M34A 1 0.114 0.002 41 4 D36A 1 0.096 0.003 17.5 0.9 T37A 1 0.208 0.007 9.4 0.4 F38A 1 ND ND ND ND L39A 1 0.31 0.03 17 1 E40A 1 0.157 0.006 37 5 H41A ND ND ND ND ND L42A 1 0.192 0.004 12.9 0.4 C43A 1 0.088 0.002 15.4 0.8 L44A 1 0.063 0.002 74 5 L45A 1 0.33 0.02 16 1 D46A 0 ND ND ND ND I47A 1 0.12 0.01 18 0.7 D48A 1 0.106 0.007 35 4 S49A 1 0.081 0.003 65 5 E50A 1 0.075 0.001 20 2 P51A 1 0.111 0.003 14.6 0.7 V52A 1 0.043 0.001 1.95 0.07 R55A 1 ND ND 3.7 0.09 S56A 1 0.084 0.002 7.7 0.3 T57A 1 0.121 0.002 42 2 S58A 1 0.031 0.001 8.1 0.3 I59A 0 ND ND ND ND I60A 1 ND ND 10.6 0.2 T62A 1 ND ND 69 5 I63A 0 ND ND ND ND P65A 1 0.11 0.007 7.7 0.4 S67A 1 0.056 0.002 12.7 0.6 R68A 1 0.063 0.002 21.2 0.8 S69A 1 0.055 0.005 27 2 V70A ND ND ND ND ND E71A 1 0.069 0.001 18.2 0.5 R72A 1 0.063 0.002 22.4 0.6 L73A 1 0.039 0.003 20 1 K74A 1 0.092 0.003 21 1 E75A 1 0.086 0.002 15.4 0.7 M76A 0 ND ND ND ND I77A 0 ND ND ND ND K78A 1 0.274 0.005 14.1 0.3 M81A 0 ND ND ND ND N82A 1 0.49 0.01 67 2 I83A 0 ND ND ND ND R85A 0 ND ND ND ND L86A 0 ND ND ND ND N87A 0 ND ND ND ND F88A 1 0.049 0.001 22.3 0.4 S89A 1 ND ND 10.1 0.3 H90A 1 0.086 0.004 4 0.2 S92A 1 0.054 0.003 13.6 0.8 H93A 1 0.076 0.002 17.3 0.5 E94A 1 0.058 0.001 12.8 0.2 Y95A 1 0.075 0.003 28 2 H96A 1 0.055 0.006 35 2 E98A 1 0.079 0.001 16.8 0.2 S99A 1 0.097 0.004 24 2 I100A 0 ND ND ND ND N102A 1 0.091 0.003 15.4 0.4 V103A 1 0.063 0.004 20 1 R104A 1 0.094 0.002 16.6 0.7 E105A 1 0.077 0.002 11.6 0.4 V107A 1 0.079 0.002 15.6 0.6 E108A 1 0.116 0.002 10.4 0.3 S109A 1 0.097 0.002 13.1 0.3 F110A 1 0.249 0.006 9.5 0.3 S113A 1 0.087 0.002 14.7 0.3 P114A 1 0.091 0.001 13.1 0.5 L115A 1 0.178 0.004 7.2 0.2 S116A 1 0.073 0.002 12.4 0.3 Y117A ND ND ND ND ND R118A 1 0.158 0.004 14.8 0.4 P119A 1 0.091 0.006 16.5 0.5 V120A 0 ND ND ND ND I122A 0 ND ND ND ND L124A 1 0.05 0.002 18.8 0.6 D125A 0 ND ND ND ND T126A 1 ND ND 44 4 K127A 1 0.05 0.001 22.5 0.8 P129A 1 0.147 0.004 15.2 0.6 E130A 1 0.061 0.002 6.5 0.2 I131A 1 0.035 0.005 53 3 R132A 0 ND ND ND ND T133A 0 ND ND ND ND I135A 1 0.082 0.001 8.5 0.2 L136A 1 0.078 0.002 11.4 0.5 Q137A 1 0.082 0.002 12.2 0.4 P140A 1 0.081 0.001 15.1 0.5 E141A 1 0.071 0.001 10.6 0.3 S142A 1 0.079 0.001 13.7 0.3 E143A 1 0.059 0.002 7.3 0.3 V144A 1 0.063 0.001 7.3 0.3 E145A 1 0.093 0.002 15.6 0.5 L146A 1 0.065 0.001 11.6 0.6 V147A 1 0.08 0.001 13.9 0.5 K148A 1 0.078 0.002 26.4 0.6 S150A 1 0.075 0.002 10.5 0.8 Q151A 1 0.077 0.002 15.2 0.6 V152A 1 0.071 0.001 16.2 0.9 L153A 1 0.076 0.001 17 0.6 V154A 1 0.08 0.002 19.4 0.7 T155A 1 0.047 0.002 5.1 0.3 V156A 1 0.071 0.002 19 1 D157A 1 0.049 0.002 6.2 0.3 P158A 1 0.081 0.001 15.8 0.7 F160A 1 0.069 0.001 13.2 0.6 R161A 1 0.043 0.002 7.6 0.4 T162A 1 0.07 0.001 16 0.6 R163A 1 0.044 0.002 5.6 0.3 N165A 1 0.074 0.001 17.3 0.7 N167A 1 0.07 0.001 14.3 0.5 T168A 1 0.076 0.002 13.3 0.6 V169A 1 0.039 0.001 7.6 0.5 W170A 0 ND ND ND ND V171A 1 0.075 0.001 18.9 0.6 D172A 1 0.088 0.004 20 1 Y173A 1 0.151 0.005 40 8 P174A 1 0.074 0.001 12.6 0.5 N175A 1 0.069 0.002 13.8 0.8 I176A 1 0.045 0.001 19.6 0.9 V177A 1 0.086 0.001 20 1 R178A 1 0.083 0.001 15.9 0.6 V179A 1 0.081 0.002 18 2 V180A 1 0.101 0.002 23 1 P181A 1 0.052 0.001 7.2 0.2 V182A 1 0.072 0.002 14.7 0.8 R185A 1 0.061 0.004 16 1 I186A 1 0.056 0.003 16 1 Y187A 1 0.056 0.002 10.6 0.8 I188A 1 0.072 0.003 14 1 D189A 1 0.069 0.002 13.1 0.5 D190A 1 0.082 0.001 22 1 L192A 1 0.088 0.002 24 1 I193A 1 0.058 0.004 43 4 S194A 1 0.085 0.001 16.1 0.5 L195A 1 0.055 0.002 16 1 V196A 1 0.075 0.002 15.4 0.8 V197A 1 0.059 0.002 12.7 0.6 Q198A 1 0.074 0.001 14 0.5 K199A 1 0.077 0.001 16.2 0.7 I200A 1 0.078 0.001 16.2 0.8 P202A 1 0.073 0.002 15 1 E203A 1 0.084 0.001 19 0.8 L205A 1 0.085 0.002 13.8 0.5 V206A 1 0.072 0.001 20 1 T207A 1 0.069 0.001 15.2 0.6 Q208A ND ND ND ND ND V209A 1 0.079 0.001 6.9 0.3 E210A 1 0.068 0.002 14 0.6 N211A 1 0.068 0.001 17.4 0.6 V214A 1 0.063 0.001 12.6 0.5 S217A 1 0.04 0.001 5.1 0.3 R218A 1 0.061 0.001 7.9 0.4 K219A 1 0.06 0.002 5.7 0.2 V221A 1 0.038 0.002 8 0.7 N222A 1 ND ND ND ND L223A 1 0.093 0.002 29 2 P224A 1 ND ND 51 3 Q227A 1 0.074 0.001 13.7 0.4 V228A 1 0.102 0.002 37 4 D229A 1 0.078 0.002 13 1 L230A 1 0.107 0.003 39 4 P231A 1 0.068 0.002 13.6 0.6 L233A 1 0.038 0.002 2.8 0.2 S234A 1 0.041 0.001 2.3 0.1 E235A 1 0.062 0.002 11.5 0.7 Q236A 1 0.061 0.001 13.5 0.8 D237A 1 ND ND ND ND V238A 1 0.039 0.002 6.6 0.3 R239A 1 0.069 0.001 14.7 0.6 D240A 1 0.04 0.001 13.9 0.7 L241A 0 ND ND ND ND R242A 1 0.057 0.001 10.4 0.4 F243A 1 0.074 0.003 28 2 V245A 1 0.056 0.001 12.9 0.7 E246A 1 0.07 0.001 15.8 0.5 H247A 1 0.001 11.8 0.5 ND V249A 1 0.06 0.002 17 1 D250A 0 ND ND ND ND I251A 1 0.083 0.002 52 1 V252A 0 ND ND ND ND F253A 0 ND ND ND ND S255A 1 ND ND 55 5 F256A 1 ND ND 46 2 V257A 1 0.061 0.003 42 5 R258A 1 0.133 0.004 23 2 K259A 1 0.122 0.002 16.4 0.7 S261A 1 0.082 0.001 22 1 D262A 0 ND ND ND ND V263A 1 0.075 0.001 21 1 V266A 1 0.08 0.002 12.8 0.5 R267A 1 0.093 0.002 17.7 0.8 L270A 1 0.072 0.002 17.8 0.6 P272A 1 0.058 0.001 10.3 0.5 E273A 1 0.045 0.001 7.1 0.3 H275A 1 0.088 0.001 15.2 0.4 I277A 1 0.067 0.002 15.7 0.7 K278A 1 0.059 0.001 17 0.9 I279A 0 ND ND ND ND I280A 1 0.05 0.004 17 2 S281A 1 0.101 0.005 30 11 K282A 0 ND ND ND ND I283A 0 ND ND ND ND E284A 0 ND ND ND ND N285A 1 0.121 0.003 48 3 H286A 1 0.088 0.001 17.4 0.2 E287A 1 0.112 0.003 32 3 V289A 1 0.412 0.003 99 5 K290A 1 0.071 0.001 15.5 0.7 R291A 1 0.116 0.003 26 1 F292A 1 0.083 0.006 50 4 D293A 1 0.113 0.004 30 2 E294A 1 0.054 0.004 12 1 I295A 0 ND ND ND ND L296A 1 0.101 0.005 54 4 E297A 1 0.063 0.002 9.8 0.7 V298A 1 0.072 0.002 16 1 S299A 1 0.084 0.002 27 1 D300A 1 0.075 0.006 15 2 I302A 1 0.045 0.001 15 0.9 M303A 1 ND ND 84 4 V304A 0 ND ND ND ND R306A 0 ND ND ND ND D308A 0 ND ND ND ND L309A 0 ND ND ND ND I311A 1 0.131 0.006 8.6 0.5 E312A 1 0.046 0.003 48 5 I313A 1 ND ND 28 2 P314A 1 0.043 0.002 15.1 0.9 E316A 1 ND ND 36 1 K317A 0 ND ND ND ND V318A 1 ND ND 28 1 F319A 1 0.179 0.004 29.5 0.7 L320A 1 0.19 0.005 17.6 0.7 Q322A 1 0.09 0.003 42 2 K323A 1 ND ND 15.8 0.3 M324A 1 0.121 0.005 47 6 M325A 1 0.131 0.002 13.1 0.4 I326A 1 0.052 0.002 17.3 0.7 R328A 1 0.073 0.002 13.2 0.4 C329A 1 0.067 0.002 50 2 N330A 1 ND ND 76 5 L331A 1 0.102 0.002 6.3 0.1 K334A 0 ND ND ND ND P335A 1 0.057 0.004 18 1 V336A 1 0.078 0.002 22.4 0.6 V337A 1 0.049 0.001 4.1 0.2 C338A 1 0.068 0.001 64 2 T340A 1 0.155 0.003 ND ND Q341A 1 0.29 0.02 22.4 0.7 M342A 1 ND ND ND ND L343A 1 0.09 0.02 16.2 0.6 E344A 1 0.14 0.001 30 2 S345A 1 0.054 0.001 11.6 0.5 M346A 1 ND ND ND ND I347A 1 0.044 0.002 25 1 T348A 1 0.074 0.003 18.7 0.4 K349A 1 0.036 0.001 18.3 0.5 R351A 1 0.055 0.002 31 2 P352A 1 ND ND 19.7 0.5 T353A 1 0.039 0.002 34 8 R354A 1 0.075 0.003 2.3 0.1 E356A 0 ND ND ND ND T357A 0 ND ND ND ND S358A 1 0.075 0.003 74 4 V360A 1 ND ND 21.7 0.8 N362A 1 ND ND 133 6 V364A 1 0.171 0.005 87 9 L365A 1 ND ND ND ND D366A 1 ND ND ND ND D369A 1 ND ND 30 1 C370A 1 0.06 0.001 20.9 0.7 I371A 1 ND ND 320 30 M372A 1 ND ND 27 2 L373A 1 0.09 0.02 50 4 S374A 1 0.065 0.007 10 1 E376A 1 ND ND 10 1 T377A 1 0.105 0.007 19 1 K379A 1 0.038 0.002 9.7 0.4 N381A 1 0.067 0.001 12.2 0.4 F382A 1 0.074 0.004 20.1 0.9 P383A 1 0.063 0.001 9 0.3 V384A 1 0.075 0.003 11.6 0.6 E385A 1 0.074 0.001 9.6 0.4 V387A 1 0.089 0.009 24 4 K388A 1 0.085 0.001 9.7 0.2 M389A 1 ND ND 61 2 Q390A 1 0.115 0.004 44 1 H391A 1 0.47 0.01 14.4 0.5 I393A 1 0.073 0.004 41 3 R395A 1 0.156 0.003 32 1 E396A 1 ND ND 99 4 E398A 1 0.15 0.01 20 2 V401A 1 0.148 0.003 8.1 0.2 Y402A 1 ND ND 49 2 H403A 1 0.064 0.001 11.2 0.3 R404A 1 0.101 0.002 17.2 0.7 Q405A 1 0.201 0.003 34 12 L406A 1 0.097 0.002 26.7 0.8 F407A 1 0.082 0.003 4 0.2 E408A 1 0.083 0.003 8.7 0.5 E409A 1 0.142 0.004 44 2 L410A 1 ND ND 20 0.6 R411A 1 0.076 0.002 27 1 R412A 1 0.073 0.002 26.5 0.9 P415A 1 0.072 0.003 20 1 L416A 1 0.047 0.002 89 4 S417A 1 0.064 0.002 57 4 R418A 1 0.12 0.003 ND ND D419A 1 ND ND ND ND P420A 1 0.08 0.005 4.8 0.4 T421A 1 0.068 0.003 3.4 0.1 E422A 1 ND ND 70 2 V423A 1 0.14 0.01 3.4 0.6 T424A 1 0.141 0.002 ND ND I426A 1 0.069 0.002 6.1 0.2 V429A 1 0.077 0.002 11.2 0.2 E430A 1 0.123 0.009 1.2 0.03 F433A 1 0.1 0.03 3.6 0.1 K434A 1 0.055 0.002 20 1 C435A 1 0.059 0.002 8.5 0.2 C436A 1 0.081 0.002 19.8 0.8 I440A 1 0.06 0.01 24 5 I441A 0 ND ND ND ND V442A 1 0.072 0.003 25 3 L443A 1 0.063 0.005 15 1 T444A 0 ND ND ND ND T445A 1 0.06 0.002 9.1 0.5 T446A 0 ND ND ND ND R448A 1 0.097 0.004 60 3 S449A 1 0.055 0.001 17 1 Q451A 1 0.076 0.002 23.1 0.8 L452A 1 0.068 0.001 3.2 0.1 L453A 1 0.048 0.002 18.4 0.7 S454A 1 0.082 0.001 15.8 0.4 R455A 1 0.104 0.001 20.5 0.6 Y456A 1 0.053 0.002 14.5 0.8 R457A 1 0.019 0.001 4.3 0.2 P458A 1 0.089 0.003 21.3 0.8 R459A 1 0.124 0.006 19 0.9 V462A 1 0.061 0.002 7.4 0.4 I463A 1 0.26 0.02 6.6 0.4 V465A 0 ND ND ND ND T466A 1 0.058 0.004 42 4 R467A 1 0.064 0.002 5.3 0.2 S468A 1 0.067 0.001 13.7 0.6 Q470A 1 0.056 0.001 10.2 0.5 R473A 1 0.059 0.002 26 4 Q474A 1 0.038 0.001 6.4 0.3 V475A 1 0.059 0.001 9.6 0.3 H476A 1 ND ND 7.5 0.5 L477A 1 0.064 0.002 14.2 0.7 C478A 1 0.042 0.005 8 1 R479A 1 0.25 0.01 9.2 0.4 V481A 1 0.077 0.003 28 1 F482A 1 ND ND 7.2 0.2 P483A 0 ND ND ND ND L484A 1 0.106 0.003 6 0.2 L485A 1 0.062 0.001 20.5 0.8 Y486A 1 0.063 0.004 26 3 R487A 1 0.062 0.001 7.1 0.3 E488A 1 0.074 0.002 14.6 0.7 P489A 1 0.075 0.002 14.7 0.6 P490A 1 0.069 0.002 13.8 0.8 E491A 1 0.066 0.001 11.3 0.4 I493A 1 0.074 0.003 32 2 W494A 1 0.21 0.01 ND ND D496A 1 0.049 0.003 3.7 0.3 D497A 0 ND ND ND ND V498A 0 ND ND ND ND D499A 1 0.08 0.02 ND ND R500A 1 0.061 0.001 18 1 R501A 1 0.089 0.002 ND ND V502A 1 0.073 0.004 25 1 Q503A 1 0.066 0.002 18.2 0.9 F504A 1 0.075 0.002 21 1 I506A 1 0.072 0.002 21 1 E507A 1 0.059 0.001 12.7 0.7 S508A 1 0.093 0.002 18.4 0.5 S509A 0 ND ND ND ND K510A 0 ND ND ND ND L511A 1 0.26 0.003 6.3 0.2 R512A 1 ND ND 7.9 0.2 F514A 1 0.308 0.006 22.6 0.7 L515A 1 0.104 0.002 21.2 0.7 R516A 1 0.072 0.002 15.3 0.6 V517A 1 0.05 0.002 13.2 0.7 D519A 1 0.121 0.006 22 1 L520A 1 0.106 0.003 11 0.9 V521A 1 0.071 0.002 25 3 I522A 1 0.082 0.005 25 1 V523A 1 0.088 0.003 31 1 V524A 1 0.167 0.004 ND ND T525A 1 0.067 0.002 32 2 W527A 1 0.32 0.03 1.5 0.2 R528A 1 0.071 0.008 9 2 P529A 1 0.085 0.008 5.7 0.3 S531A 1 0.041 0.002 8.2 0.6 Y533A 1 0.066 0.001 41 3 T534A 1 0.089 0.003 ND ND N535A 1 0.12 0.003 29 2 I536A 1 0.071 0.002 6 0.3 M537A 1 0.095 0.003 ND ND R538A 1 0.109 0.003 5.4 0.5 V539A 1 0.16 0.001 ND ND L540A 1 0.052 0.003 ND ND S541A 1 0.06 0.002 27 2 I542A 1 0.074 0.001 34 1 S543A 1 0.062 0.002 25 3